Search results for "molecular beacon"
showing 3 items of 3 documents
Molecular beacon strategies for sensing purpose
2021
Abstract The improvement of nucleic acid probes as vital molecular engineering devices will cause a noteworthy contribution to developments in bioimaging, biosensing, and disorders diagnosis. The molecular beacon (MB) which was designed by Tyagi and Kramer in 1996, are loop-stem hairpin-designed oligonucleotides armed with a quencher and a dye (also named reporter groups) at the 3′ or 5′ ends. This construction allows that MBs in the absence of their target complementary molecules do not fluoresce. Through hybridization with their specific targets a spontaneous configuration change on MBs occur and the dye and quencher separate from each other, resulting in emitting the fluorescence. MBs ar…
Optical and electrochemical DNA nanobiosensors
2011
In the past two decades, nanoscale advanced materials have been explored for biosensing molecules, so new horizons have opened up for identifying and quantifying biomolecules, and possible early diagnosis of diseases. DNA nanobiosensors show promise. This article provides an overview on their optical and electrochemical aspects. We discuss recent progress in this field, describing basic concepts of molecular beacons and quantum dots as optical nano-imaging systems. Also, carbon nanotubes provide a platform for development and advancement of electrochemical DNA nanobiosensors, which are increasingly being implemented as robust tools for detection in biomedical sciences. Refereed/Peer-reviewed
Monitoring of microRNA using molecular beacons approaches: Recent advances
2020
Abstract Micro ribose nucleic acid (miRNA) recognition is of remarkable significance in the investigation of its functions as well as diagnosis of various diseases. The significance of miRNA itself is owing to the complex regulatory roles in several physiological processes and its close association with important diseases such as cardiovascular problems, diabetes, Alzheimer's disease and different kinds of cancers. On the other hand, there are numerous challenges to conquer in the recognition of miRNA, consisting of low abundance, varied concentration range, small size, prolonged extraction process from cells and sequence resemblances. Traditional approaches for miRNA recognition do not mee…